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KMID : 0043320070300020210
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Archives of Pharmacal Research
2007 Volume.30 No. 2 p.210 ~ p.214
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Oleanolic Acid, a Pentacyclic Triterpenoid, Induces Rabbit Platelet Aggregation through a Phospholipase C-Calcium Dependent Signaling Pathway
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Lee Jung-Jin
Jin Yong-Ri
Lim Yong
Yu Ji-Yeon
Kim Tack-Joong
Yoo Hwan-Soo
Shin Hwa-Sup
Yoon Yeo-Pyo
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Abstract
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Oleanolic acid (3β-hydroxy-olea-12-en-28-oic), a pentacyclic triterpenoid, exists widely in the plant kingdom and has a wide variety of pharmacological effects such as antitumor, antifungal, insecticidal, hepatoprotective and anti-HIV activities. This paper reports that oleanolic acid induces the aggregation of rabbit platelets, a mechanism was also investigated. Oleanolic acid at concentrations of 25, 50, 100 and 200 μM induced the aggregation of washed rabbit platelets in a concentration-dependent manner. Pretreating the platelets with U73122, a phospholipase C (PLC) inhibitor, blocked the oleanolic acid induced-aggregation, whereas acetylsalicylic acid (ASA), a cyclooxygenase (COX) inhibitor, had no effect. In addition, the effect of oleanolic acid on serotonin secretion, which is a marker for dense granule secretion, was determined. Oleanolic acid induced serotonin secretion in a similar concentration-dependent manner as observed with platelet aggregation. Pretreating the platelets with U73122 blocked the oleanolic acid-induced serotonin secretion and cytosolic calcium mobilization. Overall, these results suggest that oleanolic acid can induce platelet aggregation, which may be mediated by the stimulation of PLC-mediated cytosolic calcium mobilization.
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KEYWORD
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Oleanolic acid, Platelet aggregation, Serotonin, Ca2+ mobilization
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